NV-1-488 / iFLiP Key Specs
Summary of core hardware, detection, timing, and software capabilities for the NV-1-488 / iFLiP fluorescence lifetime fiber photometry system.
Operation conditions
- Operation voltage: 110 or 220 V, preset
- Operation temperature: 18 – 25 °C (65 – 77 °F)
- Operation humidity: ≤ 70%
Light source
- Wavelength: 488 ± 10 nm
- Pulse frequency: 80 MHz
- Pulse width: ≤ 120 ps
- Power output: ≥ 50 µW (typically ≥ 100 µW)
- External intensity modulation: 0 – 5 V (5 V = minimum)
- Max modulation frequency: 1 kHz
- Light attenuation: > 20 dB (typically 25–30 dB)
- Pulse sync signal: ≥ 400 mV
Signal detector
- Single photon signal amplitude: ≥ 50 mV
- Single photon signal FWHM: 4 ns
- Quantum yield: 40%
- Dark count: ≤ 25 kCPS (typically 15 kCPS)
TCSPC lifetime measurement module
- Max photon count: 40 MCPS
- Time resolution: ≥ 25 ps
- Dead time: 25 ns
- Single photon detection: CFD
Software
- Live and post-hoc intensity display: Yes
- Live and post-hoc MPET display: Yes
- Live lifetime curve display: Yes
- Post-hoc lifetime curve fitting: Yes
- Live phasor transformation display: Yes
- Optional background subtraction: Yes
- Looping (adjustable fixed interval): Yes
Integrated system
- Number of channels: 1
- Sampling frequency: 0 – 5,000 Hz
- Continuous acquisition duration: 0 – 100 hr (limited by hard drive space and other computer physical parameters)
- System impulse response function: σ < 200 ps (of an approximating Gaussian)
- External trigger: Supported
- Recording external TTL sync signal: Supported
- Acquisition status output: TTL on during acquisition